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KMID : 0380019940090030253
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 3 p.253 ~ p.265
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Effect of Serum Type on Hybridoma Growth and Monoclonal Antibody Production
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전복환/Hwan, Chun Bok
박송용/조의철/정경환/김동일/문홍모/Park, Song Yong/Jo, Eui Cheol/Jung, Kyung Hwan/Kim, Dong Il/Moon, Hong Mo
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Abstract
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We have studied the effects of serum concentration and initial cell density on hybridoma cell growth and monoclonal antibody (MAb) production at various media supplemented with different types of serum. The types of serum were fetal bovine sera, newborn bovine calf sera, calf sera including supplemented calf sera, horse serum, and goat serum. The concentrations of each serum were 0.5, 1.25, 2.5, and 5 % (v/v) and the inoculum densities were 5 X 10°, 1 X 10;2 x 10´ cells/ml. The hybridoma cell growth and anti-Hepatitis B surface antigen (anti-HBsAg) MAb production were found to be enhanced by increasing the serum concentration and by increasing inoculum density regardless of serum type. We found that test sera purchased from different companies show different effects on cell growth and MAb production, although they are the same type of serum.
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